AbstractThe 3D architecture of the eukaryotic genome is largely shaped by cohesin complexes containing either STAG1 or STAG2 subunits. Yet, their roles in post-mitotic genome refolding remain unclear. Here, we establish STAG2 as the predominant paralog and primary orchestrator of this process. We find that upon mitotic exit, STAG1 depletion imposes negligible effects on genome refolding or tran...

AbstractMass spectrometry-based chemical proteomics enables unbiased assessment of ligand potency and selectivity across the proteome. However, current approaches remain limited by the low throughput of single-compound screening and reliance on pre-synthesized libraries. Here we devise a mechanism-driven “library-versus-proteome” platform that couples dynamic combinatorial libraries with acti...

AbstractMicrobubble cloud dynamics under short-pulse ultrasound in flowing environments remain insufficiently understood. Here, we investigate microbubble cloud behaviour in a vessel-mimicking microfluidic channel under short-pulse ultrasound (1.125 MHz) and controlled laminar flow (37.5–150 µL/min). High-speed visualization reveals two distinct regimes: an actively interacting regime characte...

AbstractObjective. Depth-of-interaction (DOI) information is essential for improving the spatial resolution in positron emission tomography (PET) imaging. Existing deep learning–based DOI discrimination methods rely on side-irradiation measurements for each detector to obtain labeled data, making them impractical for system-level calibration. Approach. This study proposes a system-level DOI di...

AbstractThe placental development requires coordinated trophoblast proliferation and differentiation, processes tightly coupled to cell cycle control. Here, we identify TMEM63B, an osmo/mechano-sensitive ion channel, as a key regulator of trophoblast cell cycle progression and placental development. TMEM63B modulates nucleocytoplasmic transport (NCT) by stabilizing NCT components, which govern ...

AbstractDuchenne muscular dystrophy (DMD) is a severe, progressive muscle-wasting disorder caused by mutations in the DMD gene, which encodes dystrophin. Although gene therapy using viral vectors has shown promise for the treatment of DMD, the clinical application of viral gene therapies is limited by vector toxicity, immunogenicity and the inability to package full-length dystrophin. Recent ad...

AbstractNumerous biomolecular condensates coexist within cells, yet the factors governing their miscibility remain poorly understood. Here, by examining 28 intrinsically disordered regions in 378 pairwise combinations, we identify key sequence determinants of condensate miscibility: serine and aromatic residues promote miscibility, while charged amino acids drive immiscibility. Mutagenesis expe...