AbstractThe clinical success of gene therapy depends critically on the development of delivery platforms capable of achieving precise, efficient and tissue-specific delivery of genetic payloads in vivo. A diverse array of carriers, including viral, non-viral, synthetic and natural vectors, have been explored to address this challenge. Among them, adeno-associated viruses, lipid nanoparticles an...

Abstract3D single-particle tracking has the potential to resolve the molecular-level forces that dictate particle motion in biological systems. However, the information gleaned from 3D single-particle tracking often cannot resolve underlying nanoscale potentials due to limited spatiotemporal resolution. To this end, we introduce an active-feedback 3D tracking microscope that utilizes silver nan...

AbstractN-terminal cysteine presents a uniquely reactive 1,2-aminothiol motif that enables site-specific modification of peptides and proteins composed solely of canonical amino acids. For both in vitro and in vivo applications, this operationally simple chemistry is an attractive alternative to bioorthogonal strategies that require noncanonical handles. However, most 1,2-aminothiol-selective r...

Highlights• Five previously uncharacterized glycofibrils are identified and structurally analyzed• The glycofibrils exhibit diverse compositions and distinct structural assemblies• TLP-2 and TLP-4a/b are glycan-coated linear strands of di- and tetra-peptide repeats• TLP-0 is a unique glycofibril composed entirely of glycans, with no protein contentTitleCryoSeek identification of glycofibril...

AbstractUltrasound mediated microbubble cavitation holds great potential for non-invasive and targeted drug delivery. However, the interplay between acoustic parameters, bubble dynamics, and resulting cellular responses remains unclear, hindering the safety improvement and optimization of the technique. This study examined the effects of ultrasound pulse sequences on microbubble dynamics and bi...

AbstractObjective. This work aims to evaluate the performance characteristics of S-positron emission tomography (PET), a compact, phoswich detector small animal PET prototype developed at Shenzhen Bay Laboratory (SZBL), in accordance with the National Electrical Manufacturers Association (NEMA) NU4-2008 protocol. Approach. The S-PET system comprises twenty flat-panel detectors arranged in two r...

AbstractCells decode mechanical cues to direct fate decisions through nuclear remodeling, yet nuclear adaptors to mechanical signals remain elusive. Here, we show that soft matrix suppresses myoblast differentiation and induces nuclear abnormality within 30 min, accompanied by a greater than 60% reduction in lamin B1 proteins levels. Mechanistically, midnolin interacts with lamin B1 and mediat...