Explore SZBL
地址：深圳市光明区光侨路高科创新中心
电话：+86-755-86967710
邮箱：webmaster@szbl.ac.cn
电话：+86-755-86967710
邮箱：webmaster@szbl.ac.cn
Nobuhiko Yamamoto, Ph.D./
Institute/Center
Institute of Neurological and Psychiatric Disorders
Email
nobuhiko(at)szbl.ac.cn
Research Direction
Cell Biology,Molecular Biology,Neuroscience
Timeline
Areas
Results
Honors
Media
Recruitment
Papers
Timeline
2024 - Present
Shenzhen Bay Laboratory
Senior Principal Investigator
2022 - 2024
Shenzhen Bay Laboratory
Visiting Principal Investigator
2002 - 2022
Grad School of Frontier Biosciences, Osaka University
Professor
1986 - 2002
Kyoto Pref University Medicine
Assistant Prof and Lecture
1982 - 1986
Department of Biophysical Engineering, Faculty of Engineering Science
Osaka University Awarded the degree of PhD in neurobiology for a thesis entitled "Studies of a neurite outgrowth-promoting factor from conditioned medium"
1980 - 1982
Awarded Master's degree in neurobiology for a study of neuronal circuits in pupil reflex
Master
1976 - 1980
Faculty of Engineering Science, Osaka University
Awarded Bachelor's degree
研究领域
Our laboratory investigates the mechanisms underlying activity-dependent gene expression involved in the formation and reorganization of neural circuits from physiological, pathological, and evolutionary perspectives. To this end, we utilize cellular biological techniques such as calcium imaging, gene expression analysis, genetic manipulation and our proprietary slice culture method.
研究成果
Dr Yamamoto obtained a PhD in Nakaakira Tsukahara lab of Osaka University by studying neurite outgrowth-promoting factor from heart cell conditioned medium. He then joined Keisuke Toyama lab in Kyoto Prefectural University of Medicine. During the period, he invented a novel organotypic culture method (Yamamoto et al., Science, 1989) and demonstrated that specific neuronal connections are established in vitro (Yamamoto et al., Neuron, 1992). In 1994 he returned to Osaka University as associate professor, and in 2002 joined Graduate School of Frontier Biosciences in Osaka University as a full professor. His lab has been mainly interested in cellular and molecular mechanisms of neuronal wiring. They have shown the mechanisms underlying formation of lamina-specific cortical connections using the in vitro approaches (Yamamoto et al., J Neurosci, 1997; J Neurosci, 2000). They have also demonstrated the role of neuronal activity in cortical circuit formation (Uesaka et al, J Neurosci, 2005, 2007; Yamada et al., PNAS, 2010) and the molecular mechanisms underlying activity-dependent axon branching by using various techniques of cellular biology, electrophysiology and molecular biology (Ohnami et al., J Neurosci, 2008; Hayano et al., PNAS, 2014, Sasaki et al., Cereb Cortex, 2020). More recently, they have shown the influence of physiological stimulation on activity-dependent gene expression (Miyasaka and Yamamoto, Frontier Neurosci, 2021), the activity-dependent dynamics of a transcription factor in cortical neurons (Kitagawa et al., J Neurosci, 2017; Atsumi et al., Cell Rep, 2024; Sugo et al., Trends Genet, 2025), the molecular mechanisms of lesion-induced axonal sprouting (Chang et al., J Neurosci, 2022), and the role of afferent-specific molecules in neocortical cytoarchitecture (Sato et al., eLife, 2022).
Honors
High-end Foreign Expert Admission Scheme (高端外国专家引进计划)
Media
Papers
1. Yamamoto N, Kurotani T, Toyama K (1989) Neural connections between the lateral geniculate nucleus and visual cortex in vitro. Science 245:192-194
2. Yamamoto N, Yamada K, Kurotani T, Toyama K (1992) Laminar specificity of extrinsic cortical connections studied in coculture preparations. Neuron 9:217-228.
3. Yamamoto N, Inui K, Matsuyama Y, Harada A, Hanamura K, Murakami F Ruthazer ES, Rutishauser U, Seki T (2000) Inhibitory mechanism by polysialic acid for lamina-specific branch formation of thalamocortical axons. J Neurosci 20:9145-9151.
4. Uesaka N, Hirai S, Maruyama T, Ruthazer E, Yamamoto N* (2005) Activity-dependence of cortical axon branch formation: A morphological and electrophysiological study using organotypic slice cultures. J Neurosci 25:1-9.
5. Yamada A, Uesaka N, Hayano Y, Tabata, T, Kano M, Yamamoto N* (2010) Role of pre- and postsynaptic activity in thalamocortical axon branching. Proc Natl Acad Sci U S A 107: 7562-7567.
6. Hayano Y, Sasaki K, Ohmura N, Takemoto M, Maeda Y, Yamashita T, Hata Y, Kitada K, Yamamoto N*. (2014) Netrin-4 regulates thalamocortical axon branching in an activity-dependent fashion. Proc Natl Acad Sci U S A 111:15226-15331.
7. Kitagawa H, Sugo N, Morimatsu M, Arai Y, Yanagida T, Yamamoto N* (2017) Activity-dependent dynamics of the transcription factor CREB in cortical neurons revealed by single-molecule imaging. J Neurosci 37:1-10.
8. Chang L, Masada M, Kojima M, Yamamoto N* (2022) Involvement of denervated midbrain-derived factors in the formation of ectopic cortico-mesencephalic project-tion after hemispherectomy. J Neurosci 42: 749-761.
9. Atsumi Y, Yamamoto N*, Sugo N* (2024) Protocol for single-molecule imaging of transcription and epigenetic factors in human neural stem cell-derived neurons. STAR Protocol 5, 103432.
10. Sugo N*, Atsumi Y, Yamamoto N* (2025) Transcription and epigenetic factor dynamics in neuronal activity-dependent gene regulation. Trends Genet, 41, 425-436.
Contact
Address: Gaoke Innovation Center,Guangming District, Shenzhen
Phone: +86-755-86967710
Email: webmaster@szbl.ac.cn
Postal Code: 518132
Copyright © 2025 Shenzhen Bay Laboratory. All Rights Reserved.